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Objective:To investigate the relationship between serum microRNA-1 (miR-1) and microRNA-155 (miR-155) expression and disease severity and prognosis of acute cerebral infarction (ACI) in patients.Methods:A total of 173 patients with ACI who received treatment in Taizhou Municipal Hospital between April 2018 and August 2019 were included in this study. These patients were divided into mild ( n = 78), moderate ( n = 54) and severe ( n = 41) groups according to the National Institutes of Health Stroke Scale (NIHSS) score. A total of 180 patients who concurrently received physical examination were included in the control group. Serum miR-1 and miR-155 expression was determined in all participants using quantitative reverse transcription-polymerase chain reaction. The included patients were divided into poor prognosis and good prognosis groups according to modified Rankin scale score within 90 days after treatment. The efficacy of serum miR-1 and miR-155 expression in the prediction of ACI prognosis and the risk factors for poor prognosis of ACI were evaluated. Results:The history of hypertension, systolic blood pressure, diastolic blood pressure, total cholesterol, low density lipoprotein cholesterol, serum miR-1 and miR-155 expression in the study group were 56.65% (98/173), (134.02 ± 27.35) mmHg, (88.45 ± 9.52) mmHg, (3.78 ± 0.82) mmol/L, (2.08 ± 0.73) mmol/L, (2.07 ± 0.37) and (1.56 ± 0.32), respectively, which were significantly higher than those in the control group [39.44% (71/180), (119.37 ± 22.14) mmHg, (81.46 ± 14.13) mmHg, (3.59 ± 0.68) mmol/L, (1.74 ± 0.69) mmol/L, (1.01 ± 0.22), (1.02 ± 0.24)], high density lipoprotein cholesterol in the study group was significantly lower than that in the control group [(1.24 ± 0.22) mmol/L vs. (1.31 ± 0.26) mmol/L, χ2 = 10.462, t = 5.542, 5.429, 2.373, 4.498, 32.865, 17.982 and 2.725, all P < 0.05]. Serum miR-1 and miR-155 expression in patients with ACI gradually increased with the increase of disease severity ( t = 10.212, 13.050, 3.092, 7.027, 3.983 and 4.099, all P < 0.05). The proportion of patients having a history of hypertension in the poor prognosis group was significantly higher than that in the good prognosis group [64.47% (49/76) vs. 42.27% (41/97), χ2 = 8.419, P < 0.05]. Systolic blood pressure, diastolic blood pressure, NIHSS score, serum miR-1 and miR-155 expression in the poor prognosis group were (136.51 ± 12.56) mmHg, (89.53 ± 6.65) mmHg, (7.26 ± 0.58) points, (1.32 ± 0.15), (1.21 ± 0.12), respectively, which were significantly higher than those in the good prognosis group [42.27% (41/97), (132.19 ± 9.32) mmHg, (86.34 ± 5.62) mmHg, (6.44 ± 0.62) points, (1.01 ± 0.07) (0.99 ± 0.05), t = 2.597, 3.418, 8.880, 10.695 and 4.633, all P < 0.05]. The receiver operating characteristic curve (ROC curve) analysis results showed the area under the curve of serum miR-1 and miR-155 expression alone in predicting ACI prognosis was 0.814 (95% CI: 0.745-0.884) and 0.839 (95% CI: 0.780-0.897), respectively. The area under the curve of miR-1 and miR-155 expression in combination in predicting ACI prognosis was 0.944 (95% CI: 0.912-0.976). Logistic regression analysis results showed that increases in admission NIHSS score, miR-1 and miR-155 expression were the risk factors for poor prognosis of ACI ( P < 0.05). Conclusion:miR-1 and miR-155 expression levels are related to the severity of ACI and therefore may be the predictors of poor prognosis of ACI.
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Objective:To summarize the genetic etiology, clinical characteristics and outcomes of neonatal hypotonia in the early stage of NICU, to provide basis for clinicians to early identify diseases and choose reasonable treatments.Methods:The clinical data of neonates with hypotonia admitted to the Department of Neonatology of Children′s Hospital of Xinjiang Uygur Autonomous Region and People′s Hospital of Xinjiang Uygur Autonomous Region from July 2017 to July 2020 were analyzed.Results:A total of 49 children were enrolled in the study, all clinically manifested as unexplained hypotonia, accompanied by special appearance 29 cases(59.18%), metabolic abnormality 18 cases(36.73%), and cranial imagin abnormality 23 cases(46.93%). After gene sequencing a, total of 22(44.89%)patients were confirmed.Thirteen (26.53%) of them were copy number variation, and gene mutation in nine cases(18.36%). The oldest age of these patients was 3 years and 2 months now, while the youngest was 4 months.A total of 16 patients were dead(32.65%). Four (8.16%) patients were lost to follow-up.At present, eighteen (62.07%) patients had mental retardation, and eleven (37.93%) of whom still existed severe physical retardation.Conclusion:We could conduct genetic testing in NICU to improve the diagnosis rate of neonates with unexplained hypotonia, which have high rate of adverse events.Neonates with a clear diagnosis should be treated promptly and give the genetic counseling to reduce the risk for the next children.
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Objective@#To investigate the clinical features of rebleeding after ruptured intracranial aneurysms.@*Methods@#A retrospective analysis was made on the diagnosis and treatment of 60 patients with ruptured intracranial aneurysms from February 2017 to February 2018 in Taizhou Hospital.According to the patients' rebleeding, the patients were divided into the control group and the observation group, with 30 cases in each group.The patients' medical records were collected.The causes, time periods, clinical manifestations and time intervals and times of rebleeding were analyzed.At the same time, the position and size of the aneurysm, the Hunt-Hess score and the combination of hypertension in the two groups were compared.@*Results@#The causes of rebleeding in the observation group were intraoperative rupture, premature movement and ambulation, defecation, abnormal mood and no obvious inducement.Preoperative rebleeding occurred in 24 cases: 19 cases before admission, 4 cases after admission, 1 case before and after admission, and 6 cases of bleeding during operation.The clinical manifestations of rebleeding in the observation group were apnea, pathological reflex, one side/bilateral pupil, light hemiplegia, meningeal irritation, and systemic convulsion and ankylosis.In the observation group, the proportions of patients with bleeding below 8d, 8-14d, 15-21d, 22-30d and more than 30d were 46.67%, 23.33%, 13.33%, 10.00% and 6.67%.There were no statistically significant differences in the position and size of the aneurysm between the two groups (all P>0.05). The Hunt-Hess scores of grade Ⅰ, Ⅱ, Ⅲ, Ⅳ in the observation group were 33.33%, 40.00%, 16.67% and 10.00%, respectively, and the Hunt-Hess scores of grade Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ in the control group was 10.00%, 36.67%, 30.00%, 20.00% and 3.33%, respectively, the difference was statistically significant (u=2.507, P=0.012). There was no statistically significant difference in the incidence of hypertension between the two groups (χ2=0.161, P>0.05).@*Conclusion@#The recurrence of intracranial aneurysms is not related to the location and size of aneurysms, and the association between hypertension and disease.It is closely related to the Hunt-Hess score.
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Objective To investigate the clinical features of rebleeding after ruptured intracranial aneurysms.Methods A retrospective analysis was made on the diagnosis and treatment of 60 patients with ruptured intracranial aneurysms from February 2017 to February 2018 in Taizhou Hospital.According to the patients＇rebleeding, the patients were divided into the control group and the observation group ,with 30 cases in each group.The patients＇medical records were collected.The causes, time periods, clinical manifestations and time intervals and times of rebleeding were analyzed.At the same time,the position and size of the aneurysm ,the Hunt -Hess score and the combination of hypertension in the two groups were compared.Results The causes of rebleeding in the observation group were intraoperative rupture , premature movement and ambulation ,defecation,abnormal mood and no obvious inducement.Preoperative rebleeding occurred in 24 cases:19 cases before admission,4 cases after admission,1 case before and after admission,and 6 cases of bleeding during operation.The clinical manifestations of rebleeding in the observation group were apnea ,pathological reflex,one side／bilateral pupil,light hemiplegia,meningeal irritation,and systemic convulsion and ankylosis.In the observation group,the proportions of patients with bleeding below 8d,8-14d,15-21d,22-30d and more than 30d were 46.67%,23.33%,13.33%,10.00% and 6.67%.There were no statistically significant differences in the position and size of the aneurysm between the two groups ( all P＞0.05). The Hunt-Hess scores of grade Ⅰ,Ⅱ,Ⅲ,Ⅳ in the observation group were 33.33%,40.00%,16.67% and 10.00%,respectively,and the Hunt -Hess scores of grade Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ in the control group was 10.00%, 36.67%,30.00%,20.00% and 3.33%,respectively,the difference was statistically significant ( u ＝2.507,P＝0.012).There was no statistically significant difference in the incidence of hypertension between the two groups (χ2 ＝0.161,P＞0.05).Conclusion The recurrence of intracranial aneurysms is not related to the location and size of aneurysms,and the association between hypertension and disease.It is closely related to the Hunt -Hess score.
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Objective To study preparation of micronized Shuanghuangpo hydrogel patch and its characteristics of transdermal penetration in vitro. Methods Micronized Shuanghuangpo hydrogel patch was prepared with some macromolecular water-soluble materials as gel base.The content of berberine was determined by HPLC method.Its transdermal penetration in vitro was determined according to the method of Chinese Pharmacopoeia 2010 edition. The rat skin penetration test in vitro was performed by modified Franz diffusion cell method. Results The hydrogel patch had constant content of berberine. Its release property in vitro conformed to Higuchi equation. The penetration of berberine in the hydrogel patch through the rat skin followed zero-order dynamics in 12 h.Its release rate was 7.934μg??(cm2)-1??h1/2 and percutaneous rate was 0.571μg??(cm2)-1??h-1. Conclusion The micronized Shuanghuangpo hydrogel patch is a new transdermal agent with sustained release property.
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Objective The purpose of the current study is to investigate the growth inhibition and the apoptosis of HeLa cells induced with TCS,and to explore the underlying molecular mechanism.Methods The antiproliferative effect of TCS on HeLa cells was measured with CCK-8.The FCM analysis was carried out to examine the effect of TCS on the cycle distribution of HeLa cells.Electron microscopy and FCM analysis were employed to observe the apoptosis of HeLa cells.The protein expression of caspase-3 was determined by Western blotting.The activity of caspase-3,8,9 was determined by a caspases colorimetric assay kit.Results 1.The proliferation of HeLa cells was significantly inhibited by TCS;2.TCS led a S-phase arrest in cell cycle;3.HeLa cell apoptosis was induced with TCS,which was characterized by chromatin condensation and apoptotic bodies.And the apoptotic rate of HeLa cells increased with time;4.The expression of caspase-3 protein was decreased significantly(P